3.1 Load images
With the Seurat object created, we are ready to load the H&E images from the file paths provided in the infoTable
. The LoadImages()
function allow you to load the images into the Seurat object and will automatically save a scaled down version of each image that you can use for plotting.
<- LoadImages(se, time.resolve = FALSE, verbose = TRUE) se
## Loading images for 2 samples:
## Reading ../data/V19D02-087_B1/spatial/tissue_hires_image.png for sample 1 ...
## Scaling down sample 1 image from 1979x2000 pixels to 400x404 pixels
## Reading ../data/V19T26-013_A1/spatial/tissue_hires_image.png for sample 1 ...
## Scaling down sample 2 image from 2000x2000 pixels to 400x400 pixels
ImagePlot(se, method = "raster", type = "raw", annotate = FALSE)

Figure 3.1: Raw H&E images