
Slide-Seq data
Last compiled: 08 September 2025
slide-seq.Rmd
In this article, we’ll demonstrate how to load Slide-Seq data with
semla
. First, we need to load a Seurat
object
with Slide-Seq data. Here we’ll use a prepared Seurat
object that can be install with the SeuratData
R
package.
See ?ReadSlideSeq
for more information on how to load
raw data.
library(semla)
library(SeuratData)
InstallData("ssHippo")
slide_seq <- LoadData("ssHippo")
Next, we can convert the Seurat
object to make it
compatible with semla
using the
UpdateSeuratForSemla
function. Note that the Slide-Seq data
doesn’t contain H&E images and therefore, all image related
functionality of semla
will be inaccessible. This includes
the Feature Viewer and image alignment tools which requires H&E
images to work.
slide_seq_semla <- UpdateSeuratForSemla(slide_seq)
Now we can use visualization functions from semla
on our
Slide-Seq data.
MapFeatures(slide_seq_semla, features = "nFeature_Spatial", colors = viridis::magma(n = 11, direction = -1)) & ThemeLegendRight()
Zoom in on Dentate gyrus:
MapFeatures(slide_seq_semla, features = "PROX1", colors = viridis::magma(n = 11, direction = -1),
crop_area = c(0.35, 0.45, 0.7, 0.7)) &
ThemeLegendRight() &
ggtitle("PROX1 expression in the Dentate Gyrus", subtitle = "")
Package versions
-
semla
: 1.4.0
Session info
## R version 4.4.2 (2024-10-31)
## Platform: aarch64-apple-darwin20.0.0
## Running under: macOS Sequoia 15.5
##
## Matrix products: default
## BLAS/LAPACK: /Users/javierescudero/miniconda3/envs/r-semlaupd/lib/libopenblas.0.dylib; LAPACK version 3.12.0
##
## locale:
## [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
##
## time zone: Europe/Stockholm
## tzcode source: system (macOS)
##
## attached base packages:
## [1] stats graphics grDevices utils datasets methods base
##
## loaded via a namespace (and not attached):
## [1] digest_0.6.37 desc_1.4.3 R6_2.6.1 fastmap_1.2.0
## [5] xfun_0.53 cachem_1.1.0 knitr_1.50 htmltools_0.5.8.1
## [9] rmarkdown_2.29 lifecycle_1.0.4 cli_3.6.4 sass_0.4.9
## [13] pkgdown_2.1.1 textshaping_0.4.0 jquerylib_0.1.4 systemfonts_1.2.1
## [17] compiler_4.4.2 rstudioapi_0.17.1 tools_4.4.2 ragg_1.3.3
## [21] bslib_0.9.0 evaluate_1.0.5 yaml_2.3.10 jsonlite_1.9.0
## [25] rlang_1.1.5 fs_1.6.5 htmlwidgets_1.6.4